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inos smad4 double immunofluorescence staining  (Proteintech)


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    Structured Review

    Proteintech inos smad4 double immunofluorescence staining
    Inos Smad4 Double Immunofluorescence Staining, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+staining/SMAD4+Antibody/pm42832089-114-5-15
    Average 96 stars, based on 161 article reviews
    inos smad4 double immunofluorescence staining - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG-HRP (Biosharp, BL003A, 1:5000) and goat anti-mouse IgG-HRP (Biosharp, BL001A, 1:5000). .. Primary cultured nucleus pulposus cells from NC and IDD groups were fixed and permeabilized, followed by double immunofluorescence staining using anti-NOTCH3 (Proteintech, 55114-1-AP) and anti-Calnexin antibodies (Santa, sc-46669). ..

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis.
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG-HRP (Biosharp, BL003A, 1:5000) and goat anti-mouse IgG-HRP (Biosharp, BL001A, 1:5000). .. Immunofluorescence Colocalization Assay Primary cultured nucleus pulposus cells from NC and IDD groups were fixed and permeabilized, followed by double immunofluorescence staining using anti-NOTCH3 (Proteintech, 55114-1-AP) and anti-Calnexin antibodies (Santa, sc-46669). ..

    Double Immunofluorescence Staining:

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG-HRP (Biosharp, BL003A, 1:5000) and goat anti-mouse IgG-HRP (Biosharp, BL001A, 1:5000). .. Primary cultured nucleus pulposus cells from NC and IDD groups were fixed and permeabilized, followed by double immunofluorescence staining using anti-NOTCH3 (Proteintech, 55114-1-AP) and anti-Calnexin antibodies (Santa, sc-46669). ..

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis.
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG-HRP (Biosharp, BL003A, 1:5000) and goat anti-mouse IgG-HRP (Biosharp, BL001A, 1:5000). .. Immunofluorescence Colocalization Assay Primary cultured nucleus pulposus cells from NC and IDD groups were fixed and permeabilized, followed by double immunofluorescence staining using anti-NOTCH3 (Proteintech, 55114-1-AP) and anti-Calnexin antibodies (Santa, sc-46669). ..

    Immunofluorescence:

    Article Title: Targeting CRBN With Thalidomide Suppresses Fibrosis After Glaucoma Drainage Surgery Through NF-κB Pathway Inhibition
    Article Snippet: Immunohistochemistry was performed using the anti-α-SMA antibody (1:100, ab5694, Abcam). .. Concurrently, CRBN was detected using immunofluorescence staining with a specific antibody (1:50, 28494-1-AP; Proteintech) in the TCT. .. Imaging was performed on the Pannoramic MIDI system (3DHISTECH, Budapest, Hungary), with three fields photographed per animal.

    Article Title: Degradation of membrane and extracellular DDR1 by a peptide-based LYTAC to overcome immune exclusion for cancer immunotherapy
    Article Snippet: Tissue immunofluorescence analysis The tumor tissues from CT26 or 4T1 tumor-bearing mice were fixed in 4% paraformaldehyde and paraffin-embedded by Servicebio (Wuhan, China). .. Immunofluorescence staining was performed using the following primary antibodies: collagen I (#66761-1-IG, Proteintech), anti-mouse DDR1 (#Abcepta, AP7660a). ..

    Article Title: FABP5 Inhibitor Attenuates HPV11 Pseudovirion Infection by Reducing Lipid Droplet Formation.
    Article Snippet: Persistent infection with low-risk human papillomavirus (HPV) can result in conditions such as verrucous hyperplasia of the skin and mucous membranes.. Among these, anogenital warts (AGW), primarily caused by HPV types 6 and 11, are common sexually transmitted infections with a high recurrence rate.. Because early infection with HPV often lacks obvious symptoms and the PsV model primarily reflects the entry phase, timely diagnosis and treatment of full HPV infection are difficult.

    Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
    Article Snippet: .. The following primary antibodies were used for immunohistochemical and immunofluorescence staining: anti-CALD1 antibody (Catalogue no. 66693-1-Ig; Proteintech Group, Chicago, IL, USA), anti-αSMA antibody (Clone no. SPM332; Novus Biologicals, Littleton, CO, USA), anti-Vimentin antibody (D21H3) XP rabbit monoclonal antibody (Catalogue no. 5741; Cell Signaling Technology, Danvers, MA, USA), anti-TAGLN antibody (Catalogue no. ab10135; Abcam, Cambridge, UK), anti-CD31 rabbit polyclonal antibody (Catalogue no. ab28364; Abcam), and anti-CD68 antibody (D4B9C) XP rabbit monoclonal antibody (Catalogue no. #76437; Cell Signaling Technology). .. For secondary detection, Alexa Fluor 488-conjugated anti-mouse IgG antibody (A11001) and Alexa Fluor 568-conjugated anti-goat IgG (H + L) Cross-Adsorbed Secondary Antibodies (A-11057) were obtained from Life Technologies (Rockville, MD, USA).

    Article Title: Unveiling the Role of Vitamin D/VDR in Promoting Endometrial Decidualization
    Article Snippet: .. After 24 h of culture, the cells were fixed with 4% paraformaldehyde and subjected to immunofluorescence staining using antivimentin (1:100, Proteintech, 10366‐1‐AP) and anticytokeratin 8 (1:100, Proteintech, 10384‐1‐AP) as primary antibodies. .. Goat antirabbit IgG secondary antibodies (Abcam, ab150077; Proteintech, SA00013‐4; 1:400) were used.

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis.
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG-HRP (Biosharp, BL003A, 1:5000) and goat anti-mouse IgG-HRP (Biosharp, BL001A, 1:5000). .. Immunofluorescence Colocalization Assay Primary cultured nucleus pulposus cells from NC and IDD groups were fixed and permeabilized, followed by double immunofluorescence staining using anti-NOTCH3 (Proteintech, 55114-1-AP) and anti-Calnexin antibodies (Santa, sc-46669). ..

    Article Title: Targeting CRBN With Thalidomide Suppresses Fibrosis After Glaucoma Drainage Surgery Through NF-κB Pathway Inhibition.
    Article Snippet: Immunohistochemistry was performed using the anti-α-SMA antibody (1:100, ab5694, Abcam). .. Concurrently, CRBN was detected using immunofluorescence staining with a specific antibody (1:50, 28494-1-AP; Proteintech) in the TCT. .. Imaging Downloaded from iovs.arvojournals.org on 03/22/2026 was performed on the Pannoramic MIDI system (3DHISTECH, Budapest, Hungary), with three fields photographed per animal.

    Staining:

    Article Title: Targeting CRBN With Thalidomide Suppresses Fibrosis After Glaucoma Drainage Surgery Through NF-κB Pathway Inhibition
    Article Snippet: Immunohistochemistry was performed using the anti-α-SMA antibody (1:100, ab5694, Abcam). .. Concurrently, CRBN was detected using immunofluorescence staining with a specific antibody (1:50, 28494-1-AP; Proteintech) in the TCT. .. Imaging was performed on the Pannoramic MIDI system (3DHISTECH, Budapest, Hungary), with three fields photographed per animal.

    Article Title: Degradation of membrane and extracellular DDR1 by a peptide-based LYTAC to overcome immune exclusion for cancer immunotherapy
    Article Snippet: Tissue immunofluorescence analysis The tumor tissues from CT26 or 4T1 tumor-bearing mice were fixed in 4% paraformaldehyde and paraffin-embedded by Servicebio (Wuhan, China). .. Immunofluorescence staining was performed using the following primary antibodies: collagen I (#66761-1-IG, Proteintech), anti-mouse DDR1 (#Abcepta, AP7660a). ..

    Article Title: FABP5 Inhibitor Attenuates HPV11 Pseudovirion Infection by Reducing Lipid Droplet Formation.
    Article Snippet: Persistent infection with low-risk human papillomavirus (HPV) can result in conditions such as verrucous hyperplasia of the skin and mucous membranes.. Among these, anogenital warts (AGW), primarily caused by HPV types 6 and 11, are common sexually transmitted infections with a high recurrence rate.. Because early infection with HPV often lacks obvious symptoms and the PsV model primarily reflects the entry phase, timely diagnosis and treatment of full HPV infection are difficult.

    Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
    Article Snippet: .. The following primary antibodies were used for immunohistochemical and immunofluorescence staining: anti-CALD1 antibody (Catalogue no. 66693-1-Ig; Proteintech Group, Chicago, IL, USA), anti-αSMA antibody (Clone no. SPM332; Novus Biologicals, Littleton, CO, USA), anti-Vimentin antibody (D21H3) XP rabbit monoclonal antibody (Catalogue no. 5741; Cell Signaling Technology, Danvers, MA, USA), anti-TAGLN antibody (Catalogue no. ab10135; Abcam, Cambridge, UK), anti-CD31 rabbit polyclonal antibody (Catalogue no. ab28364; Abcam), and anti-CD68 antibody (D4B9C) XP rabbit monoclonal antibody (Catalogue no. #76437; Cell Signaling Technology). .. For secondary detection, Alexa Fluor 488-conjugated anti-mouse IgG antibody (A11001) and Alexa Fluor 568-conjugated anti-goat IgG (H + L) Cross-Adsorbed Secondary Antibodies (A-11057) were obtained from Life Technologies (Rockville, MD, USA).

    Article Title: Unveiling the Role of Vitamin D/VDR in Promoting Endometrial Decidualization
    Article Snippet: .. After 24 h of culture, the cells were fixed with 4% paraformaldehyde and subjected to immunofluorescence staining using antivimentin (1:100, Proteintech, 10366‐1‐AP) and anticytokeratin 8 (1:100, Proteintech, 10384‐1‐AP) as primary antibodies. .. Goat antirabbit IgG secondary antibodies (Abcam, ab150077; Proteintech, SA00013‐4; 1:400) were used.

    Article Title: Targeting CRBN With Thalidomide Suppresses Fibrosis After Glaucoma Drainage Surgery Through NF-κB Pathway Inhibition.
    Article Snippet: Immunohistochemistry was performed using the anti-α-SMA antibody (1:100, ab5694, Abcam). .. Concurrently, CRBN was detected using immunofluorescence staining with a specific antibody (1:50, 28494-1-AP; Proteintech) in the TCT. .. Imaging Downloaded from iovs.arvojournals.org on 03/22/2026 was performed on the Pannoramic MIDI system (3DHISTECH, Budapest, Hungary), with three fields photographed per animal.

    Immunohistochemical staining:

    Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
    Article Snippet: .. The following primary antibodies were used for immunohistochemical and immunofluorescence staining: anti-CALD1 antibody (Catalogue no. 66693-1-Ig; Proteintech Group, Chicago, IL, USA), anti-αSMA antibody (Clone no. SPM332; Novus Biologicals, Littleton, CO, USA), anti-Vimentin antibody (D21H3) XP rabbit monoclonal antibody (Catalogue no. 5741; Cell Signaling Technology, Danvers, MA, USA), anti-TAGLN antibody (Catalogue no. ab10135; Abcam, Cambridge, UK), anti-CD31 rabbit polyclonal antibody (Catalogue no. ab28364; Abcam), and anti-CD68 antibody (D4B9C) XP rabbit monoclonal antibody (Catalogue no. #76437; Cell Signaling Technology). .. For secondary detection, Alexa Fluor 488-conjugated anti-mouse IgG antibody (A11001) and Alexa Fluor 568-conjugated anti-goat IgG (H + L) Cross-Adsorbed Secondary Antibodies (A-11057) were obtained from Life Technologies (Rockville, MD, USA).



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    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) <t>Immunofluorescence</t> staining of <t>OCN</t> and OPN in BO after the different treatments. H) Western blot of RUNX2 and <t>OCN</t> expression. I,J) Quantification of RUNX2 and <t>OCN.</t> Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) <t>Immunofluorescence</t> staining of <t>OCN</t> and OPN in BO after the different treatments. H) Western blot of RUNX2 and <t>OCN</t> expression. I,J) Quantification of RUNX2 and <t>OCN.</t> Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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    Image Search Results


    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Hydrogel-integrated osteogenic microtissues promote repair of infected intervertebral defects through sequential immunomodulation

    doi: 10.1016/j.mtbio.2026.103657

    Figure Lengend Snippet: Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Osteogenic protein expression in bone microtissues was assessed by immunofluorescence staining for OCN (ABclonal, A6205, 1:200) and OPN (ABclonal, A23658, 1:200) and by western blotting for RUNX2(Abcam, ab192256,1:1000), OCN (ABclonal, A6205, 1:1000), and GAPDH (ABclonal, AC001, 1:10,000).

    Techniques: Staining, Analysis, Activity Assay, Immunofluorescence, Western Blot, Expressing

    Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

    Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation